Impact of coenzyme Q10 on the histological structure and immunohistochemical localization of leptin in the ampulla of rat oviduct after monosodium glutamate administration

Background:The female reproductive system is very sensitive to different environmental chemicals and food additives such as monosodium glutamate. Coenzyme Q10 (CoQ10) is a naturally occurring compound and a potent antioxidant. Aim of the study:To investigate the structural changes and the immunohistochemical distribution of leptin in the ampulla of oviduct in adult female albino rats after administration of monosodium glutamate, and to study the effects of CoQ10 supplementation.Materials and methods:Fifty adult female albino rats were divided into four equal groups: group I control rats, group II receiving monosodium glutamate, group III receiving CoQ10, and group IV receiving monosodium glutamate and CoQ10. After 2 months, rats were weighed and killed during the diestrous phase. Blood samples were collected for assessment of serum cholesterol. Oviducts were prepared for histological study and immunohistochemical localization of leptin. Results:Control group showed positive immune reaction for leptin. Group II showed a significant increase in body weight and serum cholesterol associated with structural and ultrastructural changes, in addition to negative immune reaction for leptin. Group III showed similar structure to the control group. The increase in body weight and serum cholesterol in group IV was not significant. There were no changes in the histological structure of the oviduct. A positive immune reaction for leptin was detected. Conclusion:Administration of monosodium glutamate alters the histological structure and expression of leptin in the oviduct. The coadministration of CoQ10 with monosodium glutamate partially prevented these changes, suggesting a protective effect of CoQ10.