Moringa (Moringa oleifera) leaf powder in reducing the grade of liver injury of wistar rat induced by mancozeb

Background: Mancozeb (Mz) is one of the poisoning chemical substances that cause the liver injury (LI). Moringa leaf (Mo) contains antioxidant to muffle the free radical. The objective of this study is to know the effects of Mo leaf in reducing the level of LI of Wistar rat induced by Mz through an antioxidant mechanism. Method: It was an experimental study by using 24 randomized Wistar rats. Group I was terminated at the beginning of the study as the basis of necrosis index. Group II was treated as the control group. Group III was exposure with Mz started at day 11 until day 20. Group IV was given Mo started at the day 1 until day 10 and prior to giving Mz until day 20. The overall blood rats in Group II-IV were aspirated prior to and after study in measuring MDA, ALT, GPx, CAT, and SOD. The necrosis index was measured at the end of the study. The data were analyzed by unpaired t-test and linear regression. Results: There was no LI in the Group I and II. MDA level increase in Group IV (9.67 nmol/ml), but lower than Group III (45.05 nmol/ml; p = 0.006). The decrease of level of GPx, CAT, and SOD in Group IV (0.07 nmol/ml; 0.48 nmol/ml; and 81.5 U/ml), which was lower (p=0.008; p=0.004; and p=0.0001) than Group III (0.23 nmol/ml; 0.86 nmol/ml; and 140.9 U/ml). Necrosis index and ALT level increase in Group IV (4.42; 25.1 u/l) was lower (p=0.04; p=0.0001) than Group III (8.56 and 67.4 u/l). A multivariate test result that SOD was the most beneficial towards the LI status. Conclusion: Mo can reduce the LI level in Wistar rats induced by Mz through the decrease rate of endogen antioxidant of SOD.