Author/Authors :
Montazer Torbati, Fatemeh ferdowsi university of mashhad - Agriculture Faculty - Department of Animal Science, مشهد, ايران , Eftekhari Shahroudi, Fereidoun ferdowsi university of mashhad - Agriculture Faculty - Department of Animal Science, مشهد, ايران , Nassiry, Mohammad Reza ferdowsi university of mashhad - Agriculture Faculty - Department of Animal Science, مشهد, ايران , Safarnezhad, Abbas Jahad-Keshavarzi Research Center, ايران , Montazer Torbati, Mohadmmad Bagher university of tehran - Agriculture Faculty - Department of Animal Science, تهران, ايران
Abstract :
The second exon of the bovine Major Histocompatibility Complex (MHC) class II DRB3 gene was amplified by polymerase chain reaction (PCR) from 50 DNA samples of Iranian Sarabi cattle. Bovine DNA was isolated from aliquots of whole blood. A two-step polymerase chain reaction followed by digestion with restriction endonucleases RsaI, BstyI, and HaeIII was conducted on DNA from samples. Fifteen Bovine Lymphocyte Antigen (BoLA)-DRB3 alleles were assigned, including some that were only recently described for zebu cattle. Allelic frequencies ranged from 0.02 to 0.23. The most frequent alleles were *52 (frequency = 0.23), *11 (0.18) and *23 (0.15). Results of this study demonstrate that the BoLA-DRB3.2 locus is highly polymorphic in Sarabi cattles.
Keywords :
BoLA , DRB3.2 , polymerase chain reaction (PCR) , restriction fragment length polymorphism (RFLP) , Iranian Sarabi cows.
