DETERMINATION OF MYCOPHENOLIC ACID IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

A simple, sensitive and reproducible HPLC method is presented for determination of mycophenolic acid(MPA) in human plasma. Samples were prepared after precipitation of the plasma protein by addition ofacetonitrile and naproxen was used as internal standard (I.S.). Separation was performed by reversedphaseHPLC, using a Hamilton PRP-C18 Column, 51% acetonitrile and 49% potassium phosphate buffer(20 mM) at pH 3.0 as mobile phase, flow rate of 1.0 ml/min, and UV detection at 215 nm. MPA and I.S.had retention times of 7.5 and 11.35 min, respectively. The method showed an acceptable linearity in therange of 0.1μg/ml-40μg/ml with r2 of .9992. The concentration of 0.1μg/ml was determined asquantification limit. Mean absolute recovery was 94.8%. The mean intra- and inter-day reproducibility ofmethod was 4.6 and 11.4% respectively.