Author/Authors :
Jain, Darshana S SNDT Women’s University - C U Shah College of Pharmacy - Department of Pharmaceutics, India , Athawale, Rajani B SNDT Women’s University - C U Shah College of Pharmacy - Department of Pharmaceutics, India , Bajaj, Amrita N SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, India , Shrikhande, Shruti S SNDT Women’s University - C U Shah College of Pharmacy - Department of Pharmaceutics, India , Goel, Peeyush N Tata Memorial Centre - Advanced Centre for Treatment Research Education in Cancer(ACTREC) - Gude Lab, India , Nikam, Yuvraj Tata Memorial Centre - Advanced Centre for Treatment Research Education in Cancer(ACTREC) - Gude Lab, India , Gude, Rajiv P Tata Memorial Centre - Advanced Centre for Treatment Research Education in Cancer(ACTREC) - Gude Lab, India
Abstract :
Background: Nanotechnology has received great attention since a decade for the treatment of different varieties of cancer. However, there is a limited data available on the cytotoxic potential of Temozolomide (TMZ) formulations. In the current research work, an attempt has been made to understand the anti-metastatic effect of the drug after loading into PLGA nanoparticles against C6 glioma cells. Nanoparticles were prepared using solvent diffusion method and were characterized for size and morphology. Diffusion of the drug from the nanoparticles was studied by dialysis method. The designed nanoparticles were also assessed for cellular uptake using confocal microscopy and flow cytometry. Results: PLGA nanoparticles caused a sustained release of the drug and showed a higher cellular uptake. The drug formulations also affected the cellular proliferation and motility. Conclusion: PLGA coated nanoparticles prolong the activity of the loaded drug while retaining the anti-metastatic activity.
Keywords :
C6 cell line , Gliomas , PLGA nanoparticles , Temozolomide
