Author/Authors :
jecminkova, k. czech university of life sciences prague - faculty of agrobiology, food and natural resources, Czech Republic , jecminkova, k. institute of animal science, Czech Republic , kyselova, j. 2institute of animal science, Czech Republic , ahmed, a. said humboldt-universität zu berlin - albrecht daniel thaer-institute of agricultural and horticultural sciences, Germany , zavadilova, l. institute of animal science prague-uhříněves, Czech Republic , matlova, v. institute of animal science prague-uhříněves, Czech Republic , majzlik, i. czech university of life sciences prague - faculty of agrobiology, food and natural resources, Czech Republic
Abstract :
To investigate the differences between the lineages of the Czech Fleckvieh cattle in Leptin promoter SNP C963T, 695 Czech Fleckvieh cows (650 from production herds and 45 from the Genetic Resources Program (GR)) were examined using PCR-RFLP.The C and T alleles of Leptin promoter were observed with a predominance of C allele in both groups. The most frequent genotypes were CC (63%) in production herds and CT (48%) in the GR population. The present study describes, for the first time, the genetic differences in production herds and GR population in Leptin promoter C963T SNP. Variation within the Czech Fleckvieh population was observed and resulted in an advantage to the GR population. Results presented herein emphasized the importance of the GR program as a reservoir of genetic diversity for indigenous breeds.
Keywords :
single nucleotide polymorphism , cattle breeding , population diversity , leptin , PCR , RFLP
