Author/Authors :
machovič, v. university of chemistry and technology prague, Czech Republic , machovič, v. czech academy of sciences - institute of rock structure and mechanics, Czech Republic , lapčák, l. czech academy of sciences - institute of rock structure and mechanics, Czech Republic , havelcová, m. czech academy of sciences - institute of rock structure and mechanics, Czech Republic , borecká, l. czech academy of sciences - institute of rock structure and mechanics, Czech Republic , novotná, mi. university of chemistry and technology prague, Czech Republic , novotná, ma. university of chemistry and technology prague, Czech Republic , javůrková, i. university of chemistry and technology prague, Czech Republic , langrová, i. czech university of life sciences prague - faculty of agrobiology, food and natural resources, Czech Republic , hájková, š. czech university of life sciences prague - faculty of agrobiology, food and natural resources, Czech Republic , brožová, a. czech university of life sciences prague - faculty of agrobiology, food and natural resources, Czech Republic , titěra, d. bee research institute, Czech Republic , titěra, d. czech university of life sciences prague - faculty of agrobiology, food and natural resources, Czech Republic
Abstract :
The infrared (ATR-FTIR) and Raman spectroscopy was used for the structural characterization of honeybee wings. Protein, chitin, and lipid characteristic spectral features were detected using both methods. The protein secondary structure was predominantly composed of the β-sheet molecular conformation with β-turns or coil contributions. The vibration modes of the side-chain aromatic amino acid residues (tyrosine, phenylalanine, tryptophan) occurred in the wing spectra. The results of discriminant analysis showed that the infrared spectroscopy of the wing in combination with a multivariate analysis seemed promising for a resolution of the chemical structure of the wings based on lipid, proteins, and chitin content
Keywords :
vibrational spectroscopy , protein , lipid , chitin
