Author/Authors :
Amro, Amara A. King Saud University - College of Pharmacy - Department of Pharmaceutics, Saudi Arabia , Amro, Amara A. Genetic Engineering and Biotechnology Research Institute - Department of Protein Research, Egypt , Salem-Bekhit, Mounir M. King Saud University - College of Pharmacy - Department of Pharmaceutics, Saudi Arabia , Salem-Bekhit, Mounir M. Al-Azhar University - Faculty of Pharmacy - Department of Microbiology and Immunology, Egypt , Alanazi, Fars K. King Saud University - College of Pharmacy - Department of Pharmaceutics, Saudi Arabia
Abstract :
Plackett–Burman randomization method is a conventional tool for variables randomization aiming at optimization. Bacterial Ghosts (BGs) preparation has been recently established using methods other than the E lysis gene. The protocol has been based mainly on using critical concentrations from chemical compounds able to convert viable cells to BGs. The Minimum Inhibition Concentration (MIC) and the Minimum Growth Concentration (MGC) were the main guide for the BGs preparation. In this study, Escherichia coli JM109 DEC has been used to produce the BGs following the original protocol. The study contained a detail protocol for BGs preparation that could be used as a guide.
