Author/Authors :
RAFI, ABDUL The University of Agriculture - Department of Plant Pathology, Pakistan , HAMEED, ABDUL Quaid-i-Azam University - Department of Microbiology, Pakistan , AKHTAR, MUHAMMAD AFZAL NARC - Crop Disease Research Institute, Pakistan , FARHATULLAH The University of Agriculture - Department of Plant Pathology, Pakistan , SOHAIL, KAMRAN Agricultural University - Department of Plant Pathology, Pakistan , SHAHID, MUHAMMAD Agriculture Research Station (North), Pakistan , FAHIM, MUHAMMAD The University of Agriculture - Department of Plant Pathology, Pakistan
Abstract :
One hundred and twenty-five Xoo isolates were collected from twelve districts of different agro-ecological zones of Khyber Pakhtunkhwa province of Pakistan for characterization through a number of biochemical tests. HR- response was found negative for 34 isolates while 45 isolates showed negative results in terms for Pathogenecity. However, Egg yolk reaction, Tetrazolium Tolerance test, Anaerobic growth test and Oxidase Test was negative for all 125 isolates. Some of the isolates did deviate from the normal pattern and had over lapping results. Therefore, identity of the all candidate Xoo isolates was verified through Polymerase Chain Reaction (PCR).
Keywords :
Xanthomonas oryzae pv oryzae , Rice , Bacterial Leaf Blight , PCR , Biochemical characterization , Pathogenicity.
