Some Important Factors Influencing Tissue Culture Response in Wheat

Various methods and techniques have been developed and tested to improve important cereal crop species since quite long time. Among the prominent techniques, traditional plant breeding has been on leading edge. The discovery of molecular genetics and biotechnological techniques, such as genome mapping, tissue culture, genetic transformation and in vitro regeneration offered new dimensions of research to improve important crop species including wheat. The tissue culture by itself has increased the crop improvement potential to develop new cultivars with desirable traits through somaclonal and gametoclonal selection. While successful tissue culture is critical for genetic transformation, it has been facing greater impediments in wheat and related crop species. Its success depends largely upon the average effect of several important factors including explant tissue, culture medium, plant genotypes, and their interactions, the detail of which is not fully explored. Current article described this issue in detail. The information synthesized would be important to select and manipulate appropriate factor(s) for successful wheat tissue culture and regeneration that in turn would help its improvement.