Phytase production by Candida melibiosica 2491 alkalophylic strain

It has been proved that Candida melibiosica 2491 yeast strain, an intracellular phytase producer, can be well adapted to a nutritious medium with a pH range from 3.0 to 9.0. The best development of the strain was achieved at pH 8.0, which refers to the rarely encountered alkalophylic yeast strains. The maximum biosynthesis was obtained in a medium with pH of 8.5, 24th–hour aged inoculum added at concentration of 6.6% (v/v) with optical density OD600 of 0.68, aeration degree 500 dm³/dm³.h, stirring speed 300 rpm, and 24h duration of the cultivation in bioreactor. The optimal phytase activity was reached at 60°С and рН 4.5. However, over 80% of the maximum enzymatic activity was observed at the pH interval ranging from 4.0 to 8.5, which provides great potential for practical applications.