Author/Authors :
Jagadeeswaran, Murugesan Nandha College of Pharmacy - Department of Pharmaceutical Analysis, India , Gopal, Natesan Balaji Institute of Pharmaceutical Sciences - Department of Pharmaceutical Chemistry, India , Gandhimathi, Murugananthan SRIPMS, College of Pharmacy - Department of Pharmaceutical Analysis, India , Rajavel, Rajagounden Nandha College of Pharmacy - Department of Pharmaceutical Analysis, India , Ganesh, Mani Nandha College of Pharmacy - Department of Pharmaceutical Analysis, India , Sivakumar, Thangavel Nandha College of Pharmacy - Department of Pharmaceutical Analysis, India
Abstract :
A simple, rapid, reliable and accurate HPTLC method has been developed for the quantitative determination of Donepezil HCl in bulk and tablets. Various aliquots of the sample solution were spotted automatically by means of camag Linomat 5 applicator on precoated silica gel 60 F254 on aluminium sheet as stationary phase pre washed with methanol using Methanol: Chloroform (8:2 v/v) as mobile phase. The spots were scanned at 254 nm. The Rf value of Donepezil HCl was 0.54 ± 0.02. Calibration curves were linear in the range of 200-1000 ng spot^-1. The limit of detection and limit of quantification were found to be 120 ng spot^-1 and 165 ng spot^-1 respectively. The suitability of this method for the quantitative determination of compound was proved by validation in accordance with requirements of pharmaceutical regulatory standards.
