Simultaneous Spectrophotometric Estimation of Lercanidipine Hydrochloride and Atenolol in Tablet Dosage Form

Two accurate, precise, sensitive and economical spectrophotometric methods were developed and validated for simultaneous estimation of Lercanidipine hydrochloride and Atenolol in tablet dosage form. These methods were developed based on the simultaneous estimation of drugs in a binary mixture without previous separation. The methods employed were Absorbance Ratio Method (QAnalysis) (I) and Simultaneous Equation Method (Vierodt’s Method) (II). The first method employs261nm as λ1 (Isobestic point) and 273 nm as λ2 (λmax of Atenolol) for formation of equations. The second method employs estimation of a drug concentration by selecting λmax where the absorbances of these drugs were maximum. So λmax for Lercanidipine hydrochloride and Atenolol is 242 nm and 273 nm respectively. Lercanidipine hydrochloride and Atenolol obey Beer’s law in the concentration range 10-50 μg mL^-1 (r2=0.9999) and 50-250 μg mL^-1 (r2=0.9999) in 0.1 N HCL. The mean recovery for Lercanidipine hydrochloride and Atenolol were found to be 98.07±0.21% and100±0.14% from method I and 98.60±0.36% and 100±0.05% from method II. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed methods were successfully applied for simultaneous determination of Lercanidipine hydrochloride and Atenolol in routine industrial work.