Title of article :
Molecular Characterisation of Sago Starch Using Gel Permeation Chromatography Multi-Angle Laser Light Scattering
Author/Authors :
OTHMAN, ZAINON Malaysian Nuclear Agency, Malaysia , AL-ASSAF, SAPHWAN Glyndwr University - Phillips Hydrocolloids Research Centre, United Kingdom , HASSAN, OSMAN Universiti Kebangsaan Malaysia - School of Chemical Sciences and Food Technology, Faculty of Science and Technology - Food Science Programme, Malaysia
Abstract :
The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular weight Mw of native and debranched sago starch determined was 29.1 ± 2.1 × 10^6 and 1.87 ± 0.4 × 10^5 gmol^-1 respectively while radius of gyration Rg was 123.6 and 59.3 nm respectively. The reduction in Mw and Rg in debranched sago was attributed to the hydrolysis of α-1,6 glycosidic linkages by pullulanase to smaller oligosaccharides.
Keywords :
GPC , MALLS , radius of gyration , sago starch , weight , average molecular weight
