Author/Authors :
Kong, K.W. university of malaya - Faculty of Medicine - Department of Molecular Medicine, Malaysia , Khoo, H.E. Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Nutrition and Dietetics, Malaysia , Prasad, N.K. Monash University - School of Engineering, Malaysia , Chew, L.Y. Taylor’s University, Taylor’s Lakeside Campus - School of Biosciences, Malaysia , Amin, I. Universiti Putra Malaysia - Halal Products Research Institute - Laboratory of Halal Science Research, Malaysia , Amin, I. Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Nutrition and Dietetics, Malaysia
Abstract :
This study aimed to evaluate the total phenolics and antioxidant capacities of the seeds, pulp and peel of Pouteria campechiana fruit using three extraction solvents (water, 70% methanol and 70% ethanol). Among them, 70% ethanol exhibited the best solvent for yielding highest total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities. The results showed that 70% ethanol extract from the peel contained the highest TPC (2304.7 mg gallic acid equivalent/100 g dw) while the pulp has the highest TFC (6414.03 mg rutin equivalent/100 g dw). The antioxidant activities of the pulp and peel ethanolic extracts were high as determined using 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation assay (49.60 and 49.56 mmoL TE/100 g dw) and ferric reducing antioxidant power assay (43.88 and 42.94 Fe^2+/100 g dw) but not for seeds. However, their diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities were ~88%. Thus, the pulp and peel of P. campechiana fruit can be utilized as natural source for antioxidant components.
Keywords :
Antioxidant activity , Pouteria campechiana , solvent extraction , total phenolics
