Author/Authors :
QUEK, HONG KEAT Universiti Teknologi Malaysia (UTM) - Faculty of Science - Department of Chemistry, Malaysia , SANAGI, MOHD MARSIN Universiti Teknologi Malaysia (UTM) - Faculty of Science, Ibnu Sina Institute for Fundamental Science Studies - Department of Chemistry, Malaysia , WAN IBRAHIM, WAN AINI Universiti Teknologi Malaysia (UTM) - Faculty of Science, Ibnu Sina Institute for Fundamental Science Studies - Department of Chemistry, Malaysia , SALLEH, SYAIRAH Universiti Teknologi Malaysia (UTM) - Faculty of Science - Department of Chemistry, Malaysia , MISKAM, MAZIDATULAKMAM Universiti Teknologi Malaysia (UTM) - Faculty of Science - Department of Chemistry, Malaysia
Abstract :
A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15-diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good linear dynamic range between 0.7-20 μg mL^-1 with coefficients of determination (R^2) 0.9968 and acceptable limit of detection and limit of quantitation of 7 and 23 mg kg^-1, respectively, based on linearity calculations. Analyte recoveries were 90.84±27.24% for low concentration, 78.05±11.59% for medium concentration and 99.23±10.20% for high concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific migration limit of 60 mg kg^-1 (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for routine analysis for the determination of ESBO.
Keywords :
Epoxidized soybean oil , foods stored in glass jars with metal lids , gas chromatography , mass spectrometry
