Author/Authors :
Torbati, Elham islamic azad university - Microbiology Department, ايران , Bandehpour, Mojgan shahid beheshti university of medical sciences - Faculty of Medicine - Cellular and Molecular Research Center, تهران, ايران , Bandehpour, Mojgan shahid beheshti university of medical sciences - Faculty of Medicine - Biotechnology Department, تهران, ايران , Pakzad, Parviz islamic azad university - Microbiology Department, ايران , Mosaffa, Nariman shahid beheshti university of medical sciences - Faculty of Medicine - Immunology Department, تهران, ايران , Koochaki, Ameneh shahid beheshti university of medical sciences - Faculty of Medicine - Biotechnology Department, تهران, ايران , Koochaki, Ameneh shahid beheshti university of medical sciences - Faculty of Medicine - Cellular and Molecular Research Center, تهران, ايران , Kazemi, Bahram shahid beheshti university of medical sciences - Faculty of Medicine - Cellular and Molecular Research Center, تهران, ايران , Kazemi, Bahram shahid beheshti university of medical sciences - Faculty of Medicine - Biotechnology Department, تهران, ايران
Abstract :
Background: Hepatitis infection represents one of the important causes of morbidity and mortality in developing countries, however there is not any effective vaccine against hepatitis C which is one of the significant problems in vaccine project.Objectives: The aim of the present study is to evaluate the role of HCV core protein in inducing IFN-Gamma secretion and TCL activities as a vaccine in Balb/C mice.Materials and Methods: Our previous cloned plasmid (HCV Core gene into pETDuet-1) applied for protein expression in bacteria. The expressed and purified recombinant protein together with Freund’s adjuvant was injected to 15 Balb/c mice. The total IgG and IgG2a of immunized mice sera were evaluated after a week. Two weeks after booster injection,we studied the proliferation and IFNγ secretion of spleens, inguinal and popliteal lymph nodes lymphocytes by ELISA and ELISPOT.Results: The FSFC (Frequency of spot forming cells) of secreting cells of immunized mice with HCV/Core protein and sera IgG2a were considerably higher than the control groups.Conclusions: The core protein together with proper adjuvant can be a candidate vaccine against of HCV infection.
