Measurement of salusin-ß without the addition of NP-40 or Tween-20 in coronary slow-flow phenomenon

We read the study entitled “Relationship of serum salusin beta levels with coronary slow flow” by Akyüz et al. (1) with great interest. In their study, they reported that salusin-β concentrations were associated with the coronary slow-flow phenomenon. We congratulate them for their contribution to the pathophysiology of the coronary slow-flow phenomenon. However, salusins (salusin-α and salusin-β) require specific biochemistry tubes for analysis, particularly when salusin-β is analyzed in serum or plasma (2). If not, the reliability of the results is doubtful. Therefore, we wish to make the following contributions to this study conducted by Akyüz et al. (1). Salusins were discovered by Shichiri et al. (3) in 2003, and they are present in biological fluids and tissues in two forms: salusin-α (comprising 28 amino acids) and salusin-β (comprising 20 amino acids). Several studies have demonstrated that these peptides were associated with conditions such as hypertension, atherosclerotic cardiovascular disease, acute coronary syndrome, and vascular resistance (3, 4). Therefore, analyzing both salusin-α and salusin-β together while performing research on salusin will be more useful in elucidating physiopathological events