Author/Authors :
Nora-Krûkle, Zaiga Rîga Stradins University - Augusts Kirhensteins Institute of Microbiology and Virology, LATVIA , Rasa, Santa Rīga Stradiņš University - Augusts Kirhensteins Institute of Microbiology and Virology, LATVIA , Vilmane, Anda Rîga Stradins University - Augusts Kirhensteins Institute of Microbiology and Virology, LATVIA , Grâvelsina, Sabîne Rîga Stradins University - Augusts Kirhensteins Institute of Microbiology and Virology, LATVIA , Kâlis, Mârtins Rîga Stradins University - Augusts Kirhensteins Institute of Microbiology and Virology, LATVIA , Ziemele, Inga Children’s Clinical University Hospital, LATVIA , Ziemele, Inga Rîga Stradins University, LATVIA , Naciute, Milda State Research Institute - Centre for Innovative Medicine, LITHUANIA , Petraitiene, Sigita Vilnius University Hospital Santariskiu Klinikos - Children’s Hospital, Clinic of Children Diseases, Medical Faculty, LITHUANIA , Mieliauskaite, Diana State Research Institute - Centre for Innovative Medicine, LITHUANIA , Klimantaviciene, Migle Vilnius University Hospital Santariskiu Klinikos - Children’s Hospital, LITHUANIA , Girkontaite, Irute State Research Institute - Centre for Innovative Medicine, LITHUANIA , Liu, Hsin-Fu Mackay Memorial Hospital - Department of Medical Research, TAIWAN , Lin, Jih-Hui Centers for Disease Control - Center for Research, Diagnostics and Vaccine Development, TAIWAN , Lin, Yung-Cheng National Taiwan Ocean University - Institute of Bioscience and Biotechnology, TAIWAN , Chan, Hsiu-Chuan National Taiwan Ocean University - Institute of Bioscience and Biotechnology, TAIWAN , Gardovska, Dace Children’s Clinical University Hospital, LATVIA , Gardovska, Dace Rîga Stradins University, LATVIA , Murovska, Modra Rîga Stradins University - Augusts Kirhensteins Institute of Microbiology and Virology, LATVIA
Abstract :
Human bocavirus 1 (HBoV1) is a parvovirus recently found to be a possible aetiologic agent of acute respiratory disease in children. We conducted the first clinical and molecular study on this virus in Latvia (LV) and Lithuania (LT). The aim of the study was to determine the occurrence of HBoV1 in respiratory tract samples taken from hospitalised children with acute respiratory tract infections in LV and LT. In total 186 children with age one to 50 months, and who fulfilled criteria of acute respiratory tract infection, including lower respiratory tract infections, with or without fever, were included in this study. A nasopharyngeal aspirate was obtained from each patient on admission. DNA was isolated and polimerase chain reaction (PCR) performed targeting the HBoV1 NS1sequence. HBoV1 positive samples were sequenced and phylogenetic analysis was performed. HBoV1 sequence was detected in 42 (32%) of 130 LV and in 8 (14%) of 56 LT samples. In LV the majority of patients with HBoV1 infection were observed in February while in LT in October. The phylogenetic tree for HBoV1 indicated that isolates of HBoV1 cluster closely and include almost all of the isolates in this study. HBoV1 is common in Latvia and Lithuania and might be a significant pathogen that contributes to acute respiratory tract infections in children.
