Title of article :
Optimization of enzymatic hydrolysis of visceral waste proteins of beluga sturgeon Huso huso using Alcalase
Author/Authors :
Ovissipour, Mahmoudreza gorgan university of agricultural sciences and natural resources - Department of Fisheries, گرگان, ايران , Taghiof, Majid islamic azad university - Department of Chemistry, ايران , Motamedzadegan, Ali sari university of agricultural sciences and natural resources - Department of Food Science, ساري, ايران , Rasco, Barbara Washington State University - Department of Food Science and Human Nutrition, USA. , Esmaeili Molla, Abbas Shahid Rajaee Sturgeon Fish Farm, ايران
Abstract :
Fish protein hydrolysate (FPH) was produced from the viscera of beluga sturgeon (Huso huso). Using response surfacemethodology (RSM) and a factorial design to minimize enzyme use and to model the degree of hydrolysis (r2 = 0.94),the hydrolysis conditions (temperature, time and enzyme activity) were optimized. The optimum conditions were:50°C, 120 min, protease (Alcalase® 2.4 L) activity on 34 AU/kg protein. The hydrolysates of beluga visceral proteinhave a relatively high protein (66.43%) and low lipid (1.34%) content. The chemical score of the hydrolysate indicatedthat the amino acid profile of beluga sturgeon hydrolysate fulfills human adult nutritional requirements, withphenylalanine being the first limiting and predominant amino acid in the hydrolysate.
Keywords :
Fish protein hydrolysate , Sturgeon visceral protein , Alcalase , Process optimization
Journal title :
International Aquatic Research
Journal title :
International Aquatic Research
