Author/Authors :
Chai, L. C. Universiti Putra Malaysia - Faculty of Food Science and Technology, Centre of Excellence for Food Safety Research, Malaysia , Chai, L. C. Universiti Industri Selangor - Faculty of Biotechnology and Life Sciences, Malaysia , Fatimah, C. A. Universiti Industri Selangor - Faculty of Biotechnology and Life Sciences, Malaysia , Norhisyam, M. S. Universiti Industri Selangor - Faculty of Biotechnology and Life Sciences, Malaysia , Rozila, A. Universiti Industri Selangor - Faculty of Biotechnology and Life Sciences, Malaysia , Nadzirah, A. S. Universiti Industri Selangor - Faculty of Biotechnology and Life Sciences, Malaysia , Natasha, L. H. Y. Universiti Putra Malaysia - Faculty of Food Science and Technology, Center of Excellence for Food Safety Research, Malaysia
Abstract :
The objective of the present study was to develop a rapid, reliable and yet inexpensive protocol for genomic DNA extraction from frozen and ethanol-preserved Asian green-lipped mussels for random amplified microsatelite (RAM) analysis. The procedure comprised of three major steps: (1) Tissue degradation by boiling in 6% Chelex 100 resin in TE buffer; (2) Protein digestion by Proteinase K; and (3) DNA precipitation by adding 2 volumes of cold absolute ethanol. The entire procedure can be completed within two hours. The resulting RAM profiles were clear and reproducible. Our results demonstrate that the combined protocol of Chelex 100- Proteinase K-ethanol precipitation is a powerful yet economical DNA isolation method for population genetic studies involving a large sample size.
Keywords :
DNA extraction , mussel , Chelex 100 resin , RAM
