Author/Authors :
Zulkifli, Y. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Alitheen, N.B. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Son, R. Universiti Putra Malaysia - Faculty of Food Science and Technology, Centre of Excellence for Food Safety Research, Malaysia , Raha, A.R. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Samuel, L. Institute for Medical Research - Infectious Diseases Research Centre, Malaysia , Yeap, S.K. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Nishibuchi, M. Kyoto University - Center for Southeast Asian Studies, Japan
Abstract :
In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus.
Keywords :
V. parahaemolyticus , seafood , RAPD , PCR , ERIC , PCR , genomic fingerprinting
