Title of article
Cytotoxic effect of damnacanthal, nordamnacanthal, zerumbone and betulinic acid isolated from Malaysian plant sources
Author/Authors
Alitheen, N.B. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Mashitoh, A.R. Universiti Putra Malaysia - Institute of Bioscience, Malaysia , Yeap, S.K. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , Shuhaimi, M. Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , Abdul Manaf, A. Universiti Darul Iman Malaysia - Faculty of Agriculture and Biotechnology, Malaysia , Nordin, L. Universiti Putra Malaysia - Institute of Bioscience, Malaysia
From page
711
To page
719
Abstract
The present study was to evaluate the toxicity of damnacanthal, nordamnacanthal, betulinic acid andzerumbone isolated from local medicinal plants towards leukemia cell lines and immune cells by using MTT assay and flow cytometry cell cycle analysis. The results showed that damnacanthal significantly inhibited HL-60 cells, CEM-SS and WEHI-3B with the IC50 value of 4.0 μg/mL, 8.0 μg/mL and 3.3 μg/mL, respectively. Nordamnacanthal and betulinic acid showed stronger inhibition towards CEM-SS and HL-60 cells with the IC50 value of 5.7 μg/mL and 5.0 μg/mL, respectively. In contrast, Zerumbone was demonstrated to be more toxic towards those leukemia cells with the IC50 value less than 10 μg/mL. Damnacanthal, nordamnacanthal and betulinic acid were not toxic towards 3T3 and PBMC compared to doxorubicin which showed toxicity effects towards 3T3 and PBMC with the IC50 value of 3.0 μg/mL and 28.0 μg/mL, respectively. The cell cycle analysis exhibited that damnacanthal exerted its toxicity effect towards HL-60 cells by inducing apoptosis with value of 25% after 72 hours treatment. Thus, these compounds could be the potential anticancer drug with less toxic side effect.
Keywords
Betulinic acid , cytotoxic , damnacanthal , nordamnacanthal , zerumbone
Journal title
International Food Research Journal
Journal title
International Food Research Journal
Record number
2559765
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