Author/Authors :
Sahilah, A.M. Universiti Kebangsaan Malaysia - Faculty of Science and Technology, School of Chemical Sciences and Food Technology, Malaysia , Tuan Suraya, T. S. university of malaya - Faculty of Science, Institute of Biological Sciences, Malaysia , Noraida, I. university of malaya - Faculty of Science, Institute of Biological Sciences, Malaysia , Ahmad Azuhairi, A. Universiti Putra Malaysia - Faculty of Medicine and Health Science - Department of Community Health, Malaysia , Chai, L. C. Universiti Putra Malaysia - Faculty of Food Science and Technology, Centre of Excellence for Food Safety Research, Malaysia , Son, R. Universiti Putra Malaysia - Faculty of Food Science and Technology, Centre of Excellence for Food Safety Research, Malaysia
Abstract :
A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for their virulence genes and genetic diversity by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All raw vegetable isolates of C. jejuni were encoded cadF and ceuE genes. None of the C. jejuni isolates was cdtA positive. Whereas, 12 (12/20) isolates were positive for cdtB and 6 (6/20) were positive for cdtC. All the 20 isolates of C. jejuni were subtyped and they produced 18 ERIC-PCR profiles namely E1 to E18. Dendrogram performed from cluster analysis showed that the 20 isolates were grouped into 2 major clusters, cluster I and II indicating the high level of local geographical genetic variation. The detection of virulence genes among C. jejuni strains isolated from raw vegetables raised concern on the threat of campylobacteriosis.
Keywords :
Virulence genes , ERIC , PCR , raw vegetables , Campylobacter jejuni
