Author/Authors :
Lesley, M. B. Universiti Malaysia Sarawak - Faculty of Resource Science and Technology - Department of Molecular Biology, Malaysia , Velnetti, L. Universiti Malaysia Sarawak - Faculty of Resource Science and Technology - Department of Molecular Biology, Malaysia , Cheah, Y. K. Universiti Putra Malaysia - Faculty of Health Science - Department of Biomedical Science, Malaysia , Son, R. Universiti Putra Malaysia - Faculty of Food Science and Technology - Department of Food Science, Malaysia , Kasing, A. Universiti Malaysia Sarawak - Faculty of Resource Science and Technology - Department of Molecular Biology, Malaysia , Samuel, L. Universiti Malaysia Sarawak - Faculty of Resource Science and Technology - Department of Molecular Biology, Malaysia , Micky, V. Universiti Malaysia Sarawak - Faculty of Resource Science and Technology - Department of Molecular Biology, Malaysia , Nishibuchi, M. Kyoto University - Center for Southeast Asian Studies, Japan
Abstract :
A total of sixty V. parahaemolyticus strains isolated from local cockles (Anadara granosa) were investigated by their antibiotic resistance patterns and plasmid profiles. The isolates showed multiple resistances towards most of the antibiotics tested. All strains of V. parahaemolyticus isolated harbored 1-3 plasmids, with sizes ranging from 2.7 to 54 kb. All V. parahaemolyticus strains showed high multiple antibiotics in frequencies of 0.58 – 0.94 indicating that the strains were derived from high-risk sources. In addition, no particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. These findings are essential because of the suggested involvement of seafood especially shellfish and environment in transmission of this pathogen to human. Thus, indicating that seafood may be a source of food- acquired antibiotic resistant bacteria to consumer.
Keywords :
Vibrio parahaemolyticus , antibiotic resistance , plasmid profiling , cockles