Author/Authors :
Mandana, B. Universiti Putra Malaysia - Faculty of Food Science and Technology - Department of Food Technology, Malaysia , Russly, A. R. Universiti Putra Malaysia - Faculty of Food Science and Technology, Faculty of Engineering - Department of Food Technology, Department of Process and Food Engineering, Malaysia , Farah, S. T. Universiti Putra Malaysia - Faculty of Engineering - Department of Process and Food Engineering, Malaysia , Noranizan, M. A. Universiti Putra Malaysia - Faculty of Food Science and Technology - Department of Food Technology, Malaysia , Zaidul, I. S. Universiti Putra Malaysia - Faculty of Food Science and Technology - Department of Food Science, Malaysia , Ali, G. Universiti Putra Malaysia - Faculty of Food Science and Technology - Department of Food Technology, Malaysia
Abstract :
In this study, the effect of different solvent including ethanol, n-hexane and ethyl acetate on antioxidant activity and total phenolic content (TPC) of winter melon (Benincasa hispida) seeds extract was investigated using conventional Soxhlet extraction (CSE). DPPH and ABTS scavenging activity and TPC results indicated that the seed extracts obtained using ethanol possessed the highest antioxidant activity and followed by ethyl acetate and n-hexane. By considering obtained results, it was clear that there was a high positive correlation between TPC and antioxidant activity. Linoleic acid forms a significant percentage of unsaturated fatty acids of the seed extract (60.6%). It is well known that essential fatty acids including linoleic acid and linolenic acid which are detected in extracts play important roles in preventing many disease and abnormal differentiation problems. B. hispida seeds are potential source of natural antioxidant compounds to replace synthetic antioxidants.
Keywords :
Winter melon (Benincasa hispida) , conventional soxhlet extraction (CSE) , antioxidant activity , essential fatty acids
