Invitro free radical scavenging activity and bioavailability of dietary compounds caffeine, caffeic acid and their combination

A free radical scavenging activity is an important property of natural dietary compounds, caffeine and caffeic acid compound widely present in many fruits, vegetables and coffee. The present study focused on determining the antioxidant activity of caffeine, caffeic acid and (caffeine + caffeic acid) through invitro antioxidant experiments. Invitro lipid peroxidtion, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, superoxide scavenging, hydrogen peroxide scavenging, reducing power assay and total antioxidant properties. Compounds bio availability scores and structures designed by using molinspiration-cheminformatics tool and their combination (caffeine + caffeic acid) has possessed the significant free radical scavenging activity and lipid peroxidation effects in all tested methods were compared with standard antioxidant ascorbic acid. A score of bioavailability and drug likeness properties of caffeine and caffeic acid has a good score individually and the combination. Our results indicate that combination having antioxidant properties and could be used in the treatment and management of free radical mediated disease.