Effect of cardamom leaves extract as antidiabetic, weight lost and hypocholesterolemic to alloxan-induced Sprague Dawley diabetic rats

Current study was proposed to know the potency of Cardamom leaves as antidiabetic, weight lost, and hypocholesterolemic on alloxan-induced Sprague-Dawley rats. The Cardamom leaves were prepared as extract using ethanol and its termed as ethanolic cardamom leaves extract (ECLE). Thirty of 45 rats were induced with alloxan at the dose of 120 mg/kg body weight, intraperitoneal. Blood glucose level of the rats were measured after 7 days of alloxan induction and forced to fast overnight. For this purpose, we split the rats into three different groups consisted of 15 rats each. Group I; the diabetic rats were given fed added by the ECLE, Group II; the diabetic rats were only given fed, and Group III; were non diabetic rats and given only fed. Intervention was observed for 14 days, and blood sample were taken 3 times i.e.: 0, 7 and 14 days after being intervented. One mL blood was taken from eyes sub-orbital using hematocrit capillary tube then transferred to eppendorf tube containing 10% EDTA. Samples were then centrifuged at 3,000 rpm, for 10 minutes. Blood glucose and cholesterol level were quantified after blood plasma was obtained. The rats body weight were measured simultaneously with that of blood sampling. Data were then analyzed by analysis of variance, and followed by Duncan test whenever there were a 5% significant different. Data showed that blood glucose level of rats group I decreased from 201.7 to 102. 8 mg/dl (P = 0.017), cholesterol level from 77.6 to 56 mg/dl (P = 0.025), but not to the rats body weight which increased as also noted from the non-diabetic rat (P = 0.92). ECLE is potential to be used as functional food component for therapeutic of diabetic patient.