Title of article :
The Nucleocapsid Protein of Newcastle Disease Virus Promotes Solubility of the VP2 Hypervariable Region of Infectious Bursal Disease Virus in Escherichia coli
Author/Authors :
Saadun, Rafidah Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , Tan, Wen Siang Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , Omar, Abdul Rahman Universiti Putra Malaysia - Institute of Bioscience, Malaysia , Bejo, Mohd. Hair Universiti Putra Malaysia - Faculty of Veterinary Medicine - Department of Pathology and Veterinary Microbiology, Malaysia , Eshaghi, Majid Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , Yusoff, Khatijah Universiti Putra Malaysia - Institute of Bioscience, Malaysia
Abstract :
The hypervariable region (HVR) of VP2 protein of infectious bursal disease virus (lBDV) elicits neutralising antibodies, but it is highly hydrophobic and tends to form inclusion bodies when expressed in Es cherichia coli. To improve its solubility, the VP2(HVR) was fused to the C-terminal end of Newcastle disease virus (NDV) nucleocapsid (NP) protein and expressed in E. coli TOP 10 cells under the control of trc promoter. However, the fusion protein, NP-VP2(HVR)-trc, aggregated into insoluble inclusion bodies in the host cells. Therefore the coding region of NP-VP2(HVR) was sub-cloned into expression vectors containing the T7 promoter. The solubility of the NP-VP2(HVR)-T7 fusion proteins improved dramatically in E. coli BL21 (DE3), BL21 (51) and Origami B cells.
Keywords :
Newcastle disease virus , infectious bursal disease virus , VP2 solubility , hypervariable region
Journal title :
Pertanika Journal of Tropical Agricultural Science (JTAS)
Journal title :
Pertanika Journal of Tropical Agricultural Science (JTAS)
