Comparison of Anthocyanin and Phenolic Contents between Tuber and Callus of Ipomoea batatas (L.)

This study was aimed to investigate and compare the anthocyanin and phenolic contents between the tuber and callus of Ipomoea batatas (L.). Callus induction was performed in vitro using the Murashige and Skoog media supplemented with 0.5 mg/L 2,4-D and 0.1 mg/L kinetin. Meanwhile, the anthocyanin content was measured by pH differential method using cyanidin-3-glucoside as a standard. The total phenolic content was measured using the Folin-Ciocalteu method with gallic acid as a standard. Based on the results obtained, the anthocyanin content of the tuber I. batatas was 1.04 ± 0.12 mg cyanidin-3-glucoside/g fresh weight, while the anthocyanin content of the callus was 0.50 ± 0.07 mg cyanidin-3-glucoside/g fresh weight. The total phenolic content of the tuber I. batatas was 0.46 ± 0.01 mg gallic acid/g fresh weight and the phenolic content of the callus was 0.20 ± 0.01 mg gallic acid/g fresh weight. Both the anthocyanin and phenolic contents of the tuber were found to be higher than those of the callus of I. batatas