Author/Authors :
Nadeem, Ahsan University of Veterinary Animal Sciences - Department of Pathology, Pakistan , Aslam, Asim University of Veterinary Animal Sciences - Department of Pathology, Pakistan , Chaudhary, Zafar Iqbal University of Veterinary Animal Sciences - Department of Pathology, Pakistan , Ashraf, Kamran University of Veterinary Animal Sciences - Department of Parasitology, Pakistan , Saeed, Khalid University of Veterinary Animal Science - Department of Parasitology, Pakistan , Ahmad, Nisar University of Veterinary Animal Science - Department of Parasitology, Pakistan , Ahmed, Ishtiaq University of Veterinary Animal Science - University of Veterinary Animal Science, Pakistan , ur Rehman, Habib University of Veterinary Animal Science - Department of Physiology, Pakistan
Abstract :
This project was carried out to find the prevalence of trypanosomiasis in equine in District Gujranwala by using indirect fluorescent antibody technique and thin smear method. Blood samples were collected from a total of 200 horses and donkeys of different ages and either sex. Duplicate thin blood smears were prepared from each sample and remaining blood samples were centrifuged to separate the serum. Smears from each animal were processed for giemsa staining and indirect fluorescent antibody test (IFAT). Giemsa stained smears revealed Trypanosome infection in 4/200 (2.0%) samples and IFAT in 12/200 (6.0%) animals.
Keywords :
Equines , Indirect fluorescent , antibody technique , Trypanosomiasis
