Title of article
Detection of Porcine Circovirus Type 2 (PCV2) in Mosquitoes from Pig Farms by PCR
Author/Authors
Yang, Xiaohong Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China , Hou, Lidan Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China , Ye, Jing Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China , He, Qigai Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China , Cao, Shengbo Huazhong Agricultural University - College of Veterinary Medicine - State Key Laboratory of Agricultural Microbiology and Laboratory of Animal Virology, China
From page
134
To page
135
Abstract
To investigate, whether mosquitoes could be potential vectors that maintain or transmit PCV2, 59 mosquito samples were collected from suspicious PCV2-infected pig farms in Hubei and Anhui province, China. Total DNA from these mosquitoes was extracted and then tested for presence of PCV2 nucleic acid by PCR. Four (6.78%) samples showed the positive result. Subsequently, the positive PCR product was cloned into pEASY-T1 vector and sequenced. Sequence analysis displayed that homology between the PCR products and PCV2 strains were more than 94%. These results demonstrate that PCV2 nucleic acid exists in these mosquitoes, which suggests that mosquitoes could serve as mechanical transmission vectors of PCV2.
Keywords
Mosquitoes , PCV2 , Polymerase chain reaction , (PCR) , Vector
Journal title
Pakistan Veterinary Journal
Journal title
Pakistan Veterinary Journal
Record number
2562849
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