Author/Authors :
Lin, Guo-Zhen Chinese Academy of Agricultural Sciences - Lanzhou Veterinary Research Institute - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, China , Zheng, Fu-Ying Chinese Academy of Agricultural Sciences - Lanzhou Veterinary Research Institute - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, China , Zhou, Ji-Zhang Chinese Academy of Agricultural Sciences - Lanzhou Veterinary Research Institute - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, China , Wang, Guang-Hua Chinese Academy of Agricultural Sciences - Lanzhou Veterinary Research Institute - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, China , Cao, Xiao-An Chinese Academy of Agricultural Sciences - Lanzhou Veterinary Research Institute - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, China , Gong, Xiao-Wei Chinese Academy of Agricultural Sciences - Lanzhou Veterinary Research Institute - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, China , Qiu, Chang-Qing Chinese Academy of Agricultural Sciences - Lanzhou Veterinary Research Institute - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, China
Abstract :
For rapid detection of the Chlamydia psittaci abortus strain, a loop-mediated isothermal amplification (LAMP) assay was developed and evaluated in this study. The primers for the LAMP assay were designed on the basis of the main outer membrane protein (MOMP) gene sequence of C. psittaci. Analysis showed that the assay could detect the abortus strain of C. psittaci with adequate specificity. The sensitivity of the test was the same as that of the nested-conventional PCR and higher than that of chick embryo isolation. Testing of 153 samples indicated that the LAMP assay could detect the genome of the C. psittaci abortus strain effectively in clinical samples. This assay is a useful tool for rapid diagnosis of C. psittaci infection in sheep, swine and cattle.
Keywords :
Chlamydia psittaci , LAMP , MOMP gene , PCR
