Author/Authors :
Zhu, Weijuan Northeast Agricultural University - College of Veterinary Medicine, China , Ren, Yudong Northeast Agricultural University - College of Engineering, China , Li, Guangxing Northeast Agricultural University - College of Veterinary Medicine, China , Su, Dingding Hunan Agricultural University - Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Group, China , Yang, Qing Hunan Agricultural University - College of Veterinary Medicine, China , Ren, Xiaofeng Northeast Agricultural University - College of Veterinary Medicine, China
Abstract :
Viral protein 2 (VP2) of porcine parvovirus (PPV) is the major viral structural protein and responsible for eliciting neutralizing antibodies in immunized animals. In this study, the gene encoding VP2 of PPV was amplified by PCR. The VP2 gene was then cloned into the prokaryotic expression vector, pET-32a followed by expression in Escherichia coli Rosetta. The VP2 protein expression was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Rabbit polyclonal antiserum was generated using the recombinant VP2 protein. The optimal titer of the anti-VP2 antibody was determined by ELISA. The anti-VP2 antibody was able to distinguish PPV from other viruses in ELISA.
Keywords :
Antibody , ELISA , Porcine parvovirus , VP2
