Title of article :
Comparative Serological and Random Amplified Polymorphic DNA Typing for Bordetella avium Isolates in China
Author/Authors :
Yang, Ping-Ping Shandong Agricultural University - College of Animal Science and Veterinary Medicine, China , Ma, Rong-De Linqu Animal Husbandry Bureau, China , Zhao, Xue Shandong Agricultural University - College of Animal Science and Veterinary Medicine, China , Zhu, Rui-Liang Shandong Agricultural University - College of Animal Science and Veterinary Medicine, China
Abstract :
To study the similarity among Bordetella avium isolates in China, antigens and diagnostic antiserum of 22 B. avium isolates were prepared for serotyping, and a set of 20 commercially available primers was screened out to identify suitable primers for random amplified polymorphic DNA fingerprinting (RAPD) analysis in this study. Twenty-two B. avium isolates were divided into two serovars (A and B) based on their reaction in the plate-agglutination test. Four primers R1, R2, R4 and R10 resulted in informative fingerprints and were used to evaluate the B. avium isolates. Based on their RAPD patterns, a dendrogram allowed the separation of the B. avium isolates into six genetic similarity clusters. However, no direct correlation was observed between serotypes and RAPD typing among the isolates.
Keywords :
Bordetella avium , Plate , agglutination test , RAPD typing , Serotyping
Journal title :
Pakistan Veterinary Journal
Journal title :
Pakistan Veterinary Journal
