Author/Authors :
Mustafa, Riaz Huazhong Agriculture University - College of Veterinary Medicine - State Key Laboratory of Agriculture Microbiology, China , Mustafa, Riaz University of Agriculture - Faculty of Veterinary Science - Department of Pathology, Pakistan , Qi, Jingjing Huazhong Agriculture University - College of Veterinary Medicine - State Key Laboratory of Agriculture Microbiology, China , Ba, Xiaoliang Huazhong Agriculture University - College of Veterinary Medicine - State Key Laboratory of Agriculture Microbiology, China , Chen, Yingyu Huazhong Agriculture University - College of Animal Science - State Key Laboratory of Agriculture Microbiology, China , Hu, Changmin Huazhong Agriculture University - College of Veterinary Medicine - State Key Laboratory of Agriculture Microbiology, China , Liu, Xiaole Huazhong Agriculture University - College of Veterinary Medicine - State Key Laboratory of Agriculture Microbiology, China , Tu, Lingling Wuhan Keqian Animal Biological Products Co Ltd., China , Peng, Qingjie Wuhan Keqian Animal Biological Products Co Ltd., China , Chen, Huanchun Huazhong Agriculture University - College of Veterinary Medicine - State Key Laboratory of Agriculture Microbiology, China , Guo, Aizhen Huazhong Agriculture University - College of Veterinary Medicine - State Key Laboratory of Agriculture Microbiology, China
Abstract :
Mycoplasma bovis can cause different systemic problems in cattle, and recently has been resulted in huge economic losses in China. In vitro susceptibilities of 26 twice sub-cultured Chinese M. bovis field isolates were determined at physiological pH including PG45 through broth micro-dilution method. Except Huanggang isolate, all isolates and PG45 were in the sensitive range for levofloxacin, lomefloxacin and ciprofloxacin, whereas, for norfloxacin and nalidixic acid, they had shown intermediate resistant and complete resistant patterns, respectively. The multiple sequence analysis revealed point mutations in QRDRs of gyrA and parC genes of Huanggang isolate resulting in amino acid substitutions at positions 83 (S-F) in GyrA (E. coli numbering) and 80 (S-I) in ParC proteins, the latter is reported for first time in M. bovis. Conclusively, fluoroquinolones are the potential veterinary therapeutic agents for mycoplasmosis in China and resistance to these agents comes through point mutations in QRDRs of gyrA and parC genes with ParC and GyrA mutation orientation.
Keywords :
Huanggang , MICs , Mollicutes , QRDRs , Quinolones , Topoisomerases
