DETERMINATION OF ARYLOXYPHENOXY PROPIONATE GROUP OF HERBICIDES USING HPLC IN FOOD SAMPLES

A method for the separation and determination of herbicide residue, aryloxyphenoxy propionateherbicides, (fenoxaprop-p-ethyl, cyhalofop-butyl pinoxaden) in food samples with high-performance liquid chromatography detection is described. The developed method is based on microwave-assisted solvent extraction (MAE) of wheat flour and maize grains samples for 3 minutes at 550W irradiation energy. The separation was performed by using mixture of solvents acetonitrile/methanol at room temperature. The factors influencing the determination and separation, such as volume, solvents for extraction, temperature and time were optimized. Calibration curve was established using HPLC for fenoxaprop-p-ethyl and pinoxaden are 5-50 μgmL^-1 and 2.0 - 50 μgmL^-1. Recoveries in the range of 86-90% were obtained with ethanol as extraction solvent. It was observed that pinoxaden is 86% recovered in methanol and acetonitrile, respectively.