Author/Authors :
Heidari, Ahmad urmia university - Faculty of Agriculture - Department of Plant Breeding and Biotechnology, اروميه, ايران , Darvishzadeh, Reza urmia university - Faculty of Agriculture, Institute of Biotechnology - Department of Plant Breeding and Biotechnology, اروميه, ايران , Fayyaz Moghaddam, Amir urmia university - Faculty of Agriculture - Department of Plant Breeding and Biotechnology, اروميه, ايران , Rastgou, Mina urmia university - Faculty of Agriculture - Department of Plant Protection, اروميه, ايران , Hatami Maleki, Hamid university of maragheh - Faculty of Agriculture - Departrment of Agronomy and Plant Breeding, مراغه, ايران
Abstract :
Potato virus Y (PVY) as one of the serious diseases on tobacco (Nicotiana tabacum L.), reduces leaf yield and its quality. Use of resistant genotypes is being considered as the most effective, economical and a safe way to reduce damages of the virus. In the present study, the response of 90 oriental and semioriental tobacco genotypes was evaluated against PVY using randomized complete block design with three replications under controlled conditions. Each replicate consisted of 6 seedlings. The leaves of each genotype were inoculated mechanically via rubbing method at 4-6 leaf stage with PVY. Four weeks following inoculation, the studied genotypes showed different severity symptoms associated with PVY. ELISA assay were applied on the inoculated plants by the indirect method using the polyc lonal antibody specific to PVY. Optical density value of each infected sample was read at 405 nm using an ELISA reader. Analysis of variance on ELISA data showed significant differences among genotypes for concentration of PVY. Results revealed that Erzeogovina genotype with OD=0.68881 contained the highest virus concentration whereas the KB101 genotype with OD=0.0005 contained the lowest virus concentration. The differed reactions of genotypes to PVY virus encourage the use of resistance genetic resource for the reduction of disease damages.
