Author/Authors :
El Tigani-Asil, A.E. Qassim University - Faculty of Agriculture and Veterinary Medicine - Departments of Veterinary Medicine, Saudi Arabia , EL Sanousi, S.M. University of Khartoum - Faculty of Veterinary Medicine - Departments of Microbiology, Sudan , Aljameel, M.A. National Veterinary Research Corporation - Department of Pathology, Veterinary Research laboratory - Nyala, Sudan , El Beir, H. Ministry of Science and Technology - Institute of Tropical Diseases - Department of Tuberculosis, Sudan , Adam, A. University of Nyala - Faculty of Veterinary Science - Department of Pathology, Sudan , Abdallatif, M.M. North Border University - Faculty of Science - Department of Microbiology, Saudi Arabia , Hamid, M.E. King Khalid University - College of Medicine - Department of Clinical Microbiology and Parasitology, Saudi Arabia
Abstract :
This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated.
Keywords :
Acid fast bacteria , Bovine farcy , Nontuberculous mycobacteria , Pyogenic infection
