Author/Authors :
Banoee, M. tehran university of medical sciences tums - Faculty of Pharmacy - Department of Pharmaceutical Biotechnology and Medical Nanotechnology Research Centre, تهران, ايران , Banoee, M. Sciences and Research Center, Azad University, Tehran, Iran. , Banoee, M. islamic azad university - Sciences and Research Center, ايران , Mokhtari, N. islamic azad university - Department of Chemistry, ايران , Mokhtari, N. tehran university of medical sciences tums - Faculty of Pharmacy - Department of Pharmaceutical Biotechnology and Medical Nanotechnology Research Centre, تهران, ايران , Akhavan Sepahi, A. islamic azad university - Sciences and Research Center, ايران , Jafari Fesharaki, P. tehran university of medical sciences tums - Faculty of Pharmacy - Department of Pharmaceutical Biotechnology and Medical Nanotechnology Research Centre, تهران, ايران , Jafari Fesharaki, P. islamic azad university - Sciences and Research Center, ايران , Monsef-Esfahani, H. R. tehran university of medical sciences tums - Faculty of Pharmacy - Department of Pharmacognosy, تهران, ايران , Ehsanfar, Z. tehran university of medical sciences tums - Faculty of Pharmacy - Department of Pharmaceutical Biotechnology and Medical Nanotechnology Research Centre, تهران, ايران , Khoshayand, M.R. tehran university of medical sciences tums - Faculty of Pharmacy, and Pharmaceutical Sciences - Department of Food and Drug Control, تهران, ايران , Shahverdi, A. R. tehran university of medical sciences tums - Faculty of Pharmacy - Department of Pharmaceutical Biotechnology and Medical Nanotechnology Research Centre, تهران, ايران
Abstract :
In this research the ethanol extract ofblack tea and its tannin free fraction used for green synthesis of gold nanoparticles. All the extracts were used separately for the synthesis of gold nanoparticles through the reduction of aqueous AuCl^-4. Transmission electron microscopy and visible absorption spectroscopy confirmed the reduction of gold ions to gold nanoparticles. The ethanol extract of black tea and its tannin free ethanol extract produced gold nanoparticles in the size ranges of 2.5-27.5 nm and 1.25-17.5 nm with an average size of 10 nm and 3 nm, respectively. The prepared colloid gold nanoparticles, using the ethanol extract of black tea, did not show the appropriate stability during storage time (24 hours) at 4°C. In contrast, gold colloids, which were synthesized by a tannin free fraction showed no particle aggregation during short and long storage times at the same conditions. To the best of our knowledge, this is the first report on the rapid synthesis of gold nanoparticles using ethanol extract of black tea and its tannin free fraction.
Keywords :
Camellia sinensis , Gold nanoparticles , Synthesis , Tannin , Black tea , Chloroauric acid
