Author/Authors :
Homayouni Moghadam, Farshad yazd shahid sadoghi university of medical sciences - Neurobiomedical Research Center, School of Medicine - Department of Physiology, ايران , Tayebi, Tahereh yazd shahid sadoghi university of medical sciences - School of Medicine - Department of Physiology, ايران , Dehghan, Maryam yazd shahid sadoghi university of medical sciences - Neurobiomedical Research Center, School of Medicine, ايران , Eslami, Gilda yazd shahid sadoghi university of medical sciences - School of Medicine - Department of Medical Parasitology and Mycology, ايران , Nadri, Hamid yazd shahid sadoghi university of medical sciences - School of Pharmacy - Department of Medicinal Chemistry, ايران , Moradi, Alireza yazd shahid sadoghi university of medical sciences - School of Pharmacy - Department of Medicinal Chemistry, ايران , Vahedian-Ardakani, Hassanali yazd shahid sadoghi university of medical sciences - School of Medicine - Department of Internal Medicine, ايران , Barzegar, Kazem yazd shahid sadoghi university of medical sciences - School of Medicine - English Language Department, ايران
Abstract :
Background: Bone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body. MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical factors such as pH could recommend new approaches for preparation of suitable scaffolds for bone and cartilage tissue engineering. In present study, the effect of alkaline medium on chondrogenic and osteogenic differentiation of rat MSCs was evaluated Methods: MSCs were harvested from bone marrow of animals and then the response of passage1 and 2 of MSCs (P1 MSCs P2 MSCs) to the culture in alkaline medium (pH: 8) was evaluated. Cytochemical and immunocytochemical staining were performed to distinguish chondrocytes and osteocytes. Real-time PCR was performed to evaluate the type II collagen and osteopontin mRNA levels. Results: Staining for type II collagen, a chondrocytic specific marker, revealed that after one-week culture in alkaline medium, a considerable amount of P1 MSCs had shown chondrocytic morphology. By prolonging the culture period up to 4 weeks, osteogenic cells with expanded matrix and mineralized areas around them were appeared. Results of real-time PCR showed that P1 MSCs after one week culture in alkaline medium expressed highest rate of type II collagen and osteopontin mRNA among all groups. Conclusion: This study demonstrated that alkaline medium is a potent chondrogenic differentiation inducer for MSCs in their first passage.
Keywords :
Alkaline medium , Chondrocyte , Mesenchymal stem cell , Osteoblast
