Title of article :
The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells
Author/Authors :
Baharara, Javad islamic azad university - Research Center for Animal Development Applied Biology - Department of Biology, ايران , Amini, Elaheh kharazmi university (university of tarbiat moallem) - Faculty of Biological Sciences - Developmental Animal Biolog, تهران, ايران , Kerachian, Mohammad Amin mashhad university of medical sciences - Medical Genetic Center, Faculty of Medicine - Department of Medical Genetics, ايران , Soltani, Mozhgan islamic azad university - Department of Biology, ايران
Abstract :
Objective(s): Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage.Materials and Methods: Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concentration of extract. After 21 days, Alizarin red staining, alkaline phosphatase assay and RT-PCR were performed. The results were analyzed by ANOVA software and P value 0.05 was considered significant.Results: Morphological methods revealed that appropriate concentrations of extract increased osteogenic differentiation in a dose-dependent manner. RT-PCR revealed that extract without or with osteogenic medium due to osteopontin expression had a potential role in osteogenesis.Conclusion: Based on our data it concluded that S. cucumber extract stimulated Bone marrow mesenchymal cells to differentiate into osteogenic lineage without existence of osteogenic medium.
Keywords :
Bone marrow , Differentiation , Marine , Osteogenic
Journal title :
Iranian Journal of Basic Medical Sciences
Journal title :
Iranian Journal of Basic Medical Sciences
