Author/Authors :
BEGUM, SHAHANARA Rajshahi Medical College - Department of Microbiology, Bangladesh , SAMSUZZAMAN, M. D. University of Rajshahi - Department of Biochemistry and Molecular Biology, Bangladesh , AHMED, IFTIKHAR Rajshahi Medical College - Department of Microbiology, Bangladesh , HASSAN, PARVEZ University of Rajshahi - Institute of Biological Sciences, Bangladesh , ABSAR, NURUL University of Rajshahi - Department of Biochemistry and Molecular Biology, Bangladesh , HAQ, JALALUDDIN A. BIRDEM - Department of Microbiology, Bangladesh
Abstract :
The action of purified intracellular toxin (PIT) from Pseudomonas aeruginosa was examined in brine shrimp lethality bioassay. The LC50 of the PIT was calculated to be 25 μg/ml. The PIT agglutinated both albino rat and rabbit erythrocytes more potently than did extracellular crude toxin (ECT). Galactose and D-mannose, however, inhibited the agglutination property of PIT and ECT respectively. Intradermal injection of PIT caused changes on the tissues of rabbit skin at a lower dose than that of ECT.
Keywords :
Pseudomonas aeruginosa toxin , Brine shrimp nauplli , Hemagglutination , Effect on rabbit tissue.
