Author/Authors :
Konjhodzic, Rijad University of Sarajevo - Clinical Centre - Clinical Pathology, Bosnia and Herzegovina , Dervovic, Edina University of Sarajevo - Clinical Centre - Clinical Microbiology, Bosnia and Herzegovina , Kurtovic-Basic, Ilvana University of Sarajevo - Clinical Centre - Clinical Pathology, Bosnia and Herzegovina , Stomornjak-Vukadin, Meliha University of Sarajevo - Clinical Centre - Clinical Pathology, Bosnia and Herzegovina , Muhic, Adis University of Sarajevo - Clinical Centre - Clinical Pathology, Bosnia and Herzegovina , Baljevic, Sumeja University of Sarajevo - Clinical Centre - Clinical Pathology, Bosnia and Herzegovina , Pirnat-Gegic, Aida University of Sarajevo - Clinical Centre - Clinical Pathology, Bosnia and Herzegovina , Basic, Ejub Sarajevo University - Clinical Center - Clinic for Gynecology and Obstetrics, Bosnia and Herzegovina , Bilalovic, Nurija University of Sarajevo - Clinical Centre - Clinical Pathology, Bosnia and Herzegovina
Abstract :
Introduction: QF PCR has recently entered diagnostic practice as a possible way to bypass culturing of the fetal cells, as well as to provide a rapid resporise following anmiocentesis. Material and methods: The effective value of the QF PCR remains a much debated issue, positions ranging from that it makes classic kayotyping obsolete except in special occasions, to that it is no more than a guideline for a mandatory karyotype. Current practices of the. gynecology specialists generates samples in such fashion that kariotyping of samples quickly falls behind to the point of obsoleteness, because, by the time a karyotype has been finished, a window of opportunity for termination of pregnancy has closed. Results: QF PCR provides a rapid response alternative, but it is necessary to establish its reproducibility, as well as an algorithm of its use along classic kariotyping. This study contains sarnple5 processed in a period from August l, 2012 to December 31 2013 in both QF PCR and classic karyotype. Object of this study was compare results obtained by two methods, and establish confidence interval of the QF PCR testing. Overall, 661 anmiotic fluid samples were processed and typed with QF PCR, out of which 221 were done in parallel with karyiotyping, as an confirmation of results.
