Aquaporin10 Expression in Cadaveric Ileum with Varying Degrees of Mucosal Injury

Objective: This study was conducted to provide an evidence for the qualitative analysis of AQP10 expression in cadaveric ileum and the mucosal changes that occur within first four hours of death. Study Design: Descriptive Study. Place and Duration: The study was conducted at Forensic Department of King Edward Medical University, Lahore. Materials and Methods: 30 specimens were collected from cadavers having an apparently healthy non-diseased intestine. Specimens of small intestine from cadavers with any obvious disease such as ulcers, injuries, deformities, obstruction due to scarring, cancerous states, perforations, tuberculosis and those who were shifted to the cold storage after 4 hours or more after death were excluded. All the specimens were immunostained with Fast Red TR / naphthol AS-MX Reagent. Variables studied were the presence/absence of AQP-10 and degree of mucosal injury. Results: All cases showed absence of aquaporin10 staining irrespective of any degree of mucosal injury. Conclusion: Cadaveric ileum showed absence of aquaporin10 which may be due to ischemia produced after death and the mucosal insult of varying degrees.