Author/Authors :
Sarvari, Jamal shiraz university of medical sciences - School of Medicine - Department of Bacteriology and Virology, ايران , Mojtahedi, Zahra shiraz university of medical sciences - Institute for Cancer Research, School of Medicine, ايران , Kuramitsu, Yasuhiro Yamaguchi University Graduate School of Medicine - Department of Biochemistry and Functional Proteomics, Japan , Fattahi, Mohammad Reza shiraz university of medical sciences - School of Medicine - Department of Internal Medicine, ايران , Ghaderi, Abbas shiraz university of medical sciences - Institute for Cancer Research,School of Medicine, ايران , Nakamura, Kazuyuki Yamaguchi University Graduate School of Medicine - Department of Biochemistry and Functional Proteomics, Japan , Erfani, Nasrollah shiraz university of medical sciences - Institute for Cancer Research,School of Medicine, ايران
Abstract :
Background: Hepatocellular carcinoma (HCC), a major fatal cancer worldwide, is induced by different etiological factors in the liver. Objectives: To gain insight into serum protein profiling of HCC with different etiologies. Patients and Methods: We subjected the sera of HBV-HCC, HCV-HCC, non-B non-C-HCC patients, and healthy volunteers to two-dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS). Results: We found 30 differentially expressed protein spots (≥ 1.5 fold P 0.05) between these two analyses; of them 17 protein spots corresponding to 8 proteins were identified by MS. Transthyretin, leucine rich α-2-glycoprotein, and ficolin 3 were differentially expressed between HBV-related HCC and non-B non-C-HCC sera. Moreover, haptoglobin α-2 isoforms were decreased in HCV-HCC compared to non-B non-CHCC. Conclusions: Serum proteome analyses of HCC with different origins showed a differential protein pattern, presumably related to different hepatopathogenesis in liver induced by different agents. Further studies are required to clarify the importance of identified proteins for early diagnosis of HCC with different origins.
Keywords :
Hepatitis B Virus , Hepatitis C , Carcinoma, Hepatocellular , Electrophoresis, Gel, Two , Dimensional , Biological Markers