Author/Authors :
ISMAIL, MOHD ISWADI Universiti Kebangsaan Malaysia - Faculty of Medicine - Department of Physiology, Malaysia , OSMAN, KHAIRUL Universiti Kebangsaan Malaysia - Faculty of Allied Health Sciences - Forensic Science Programme, Malaysia , KING, ONG HUA Kolej Universiti Teknologi Pengurusan Malaysia - School of Health Sciences - Department of Biomedical Science, Malaysia , HASSAN, NURHASLINA Kolej Universiti Teknologi Pengurusan Malaysia - School of Health Sciences - Department of Biomedical Science, Malaysia , ELIAS, EZLAN Universiti Industri Selangor - Faculty of Biomedical Health Science, Malaysia , AMBIA, KASWANDI MD. Universiti Kebangsaan Malaysia - Faculty of Allied Health Sciences - Department of Biomedical Science, Malaysia , GHAZALI, AHMAD ROHI Universiti Kebangsaan Malaysia - Faculty of Allied Health Sciences - Department of Biomedical Sciences, Malaysia , MOHAMED, JAMALUDIN Universiti Kebangsaan Malaysia - Faculty of Allied Health Sciences - Department of Biomedical Sciences, Malaysia , OMAR, BAHARUDDIN HJ Universiti Kebangsaan Malaysia - Faculty of Allied Health Sciences - Department of Biomedical Science, Malaysia
Abstract :
Forensic entomology is defined as knowledge about insect and its relationship with a decomposed human body. With this knowledge, post-mortem interval (PMI) can be estimated. PMI can be determined by taking into consideration the insect species and the developmental stage of the insects. Identification of the insect species requires the insect to develop into adulthood. Since this will take a relatively long time, the objectives of this study were to optimize temperature and humidity for the growth of Chrysomya megacephala larvae to adults. C. megacephala larvae were transferred into a rearing container and put inside a special incubator with temperature adjusted to 27, 30, 33, 36 and 39°C separately. Once optimum temperature for larvae growth was determined, optimum relative humidity was determined then for the length of time taken for C. megacephala larvae to develop into adults. To achieve this, the larvae of C. megacephala were incubated in a special incubator and the relative humidity set at 54.2, 57.6, 76.0 and 67.5% (control) separately. The developmental stages of C. megacephala for both temperatures and humidity levels were recorded accordingly. Results obtained indicated that C. megacephala developmental stages grew much faster in 33oC than other temperatures. The optimum relative humidity level for the species was 76.0%. By utilizing the appropriate temperature and relative humidity the development of C. megacephala, from eggs to adults could be reduced from 8 to 9 days to 5 days.
