Assessment of the Anticancer and Antigenotoxic potential of Costus speciosus extract against HCT116 cell line

The aim of the present study was to analyze different effect of the costus speciosus extract against cancer cell line and to study its genotoxicity activity. The wholeplant extract was compare to most effective and known compound diosgnen. For thispurpose, antiproliferation and antigrowth activity was determined using cell viabilityassessment assays such as WST-1 assay and trypan blue dye exclusion staining assaysagainst human colon tumor cell line (HCT116). Acridine orange dye and Erythrosine-B dyewas used to understand the mechanism, whether the death of cells is due to necrosis orApoptosis. Furthermore, Comet assay and Ames test were used for the detection of genetictoxicity of whole plant material. Our results showed that with concentration of 300 μg/ml ofwhole plant extract almost showed 100 % of cell suppression in HCT116 cell line. This datawas also confirmed that the extract contained some compounds other than Diosgnen, whichcan suppress the growth of the cells. Therefore, we might concluded that, the whole plant ofcostus speciosus contains active compounds other than Diosgnen, which has the ability tokill cancer cells.