Alalysis of Single Nucleotide Polymorphism (SNP) in the flanking Region of the CD14 Gene (C-159t) in the relation to Neonatal Sepsis in Egypt

Sepsis is a persistent and vexing problem in neonates. Genetic variation in the innate immune system of the host may play a role in determining the risk of developing and outcome from infection. Variation in the ability to recognize pathogens may influence the risk of infection. One of the primary molecules that function in recognition of pathogens is CD 14. The purpose of this study was to verify association of CD 14 C-159T single nucleotide polymorphism (SNP) in blood of 40 septic and 20 control neonates. Analysis of polymorphism was performed using the technique polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Genotype analysis revealed 3 genotypes TT, CC, TC. There was a statistical significant higher rate ofTTand lower CC genotypes in septic neonates as compared to control group (p = 0.03, OR = 4.18 and 95% CI 1.13 to 15.42). It was found that TT genotype was 47.5% and associated with increase level of sCD 14 together with decreased mCD14 expression with highly statistical significant difference. As there were significant results with TT genotype in relation to sepsis, it was verified that TT genotype represents risk factor of sepsis. In conclusion, these data provide insight into the pathogenic role of the CD 14C-159T polymorphism in the pathogenesis of sepsis as -159TT may favour increase risk of acquiring infections.