Author/Authors :
Mohammad Beigi, Sepideh Center of Excellence in Electrochemistry - School of Chemistry - College of Science - University of Tehran, Tehran, Iran , Ahmed Khurshid, Chrow College of Pharmacy - University of Kirkuk, Iraq , Mesgari, Fazeleh Center of Excellence in Electrochemistry - School of Chemistry - College of Science - University of Tehran, Tehran, Iran , Poursaberi, Tahereh Analytical Chemistry Research Center - Research Institute of Petroleum Industry, Tehran, Iran , Hosseini, Morteza Department of Life Science Engineering - Faculty of New Sciences & Technologies - University of Tehran, Tehran, Iran
Abstract :
After modifying an electrogenerated chemiluminescence sensor with a
Ru(bpy)32+/Sm2O3 Nanoparticles(NPs)/chitosan composite, the resulting electrode was used as
a sensitive tool for the detection of phenylalanine. The electrochemiluminescence (ECL) signal resulting from the electrochemical interaction between tris(2,2-bipyridyl) ruthenium(II)
(Ru(bpy)32+) and the analyte on the surface of the modified sensor. The presence of the Sm2O3NPs was found to enhance the ECL signal. Further the influences of Ru(bpy)32+
concentration, potential scan rate and solution pH on the intensity of the signal were monitored and optimized. The method showed a linear response in the range of 5.0×10-11 to 5.0×10-7 M
(R2=0.9824) with a detection limit of 1.4×10-11 M. The modified ECL sensor was further used for the analysis of phenylalanine in human serum samples and an RSDs of 4.4% was observed, proving the high sensitivity, selectivity, and good stability of the sensor.
Keywords :
Electrochemiluminescence , Phenylalanine , Samarium , Enhance
